[solved] Problem sending test E-Mail

Can you send email directly from docker server (not from inside container)? If it is linux: something around:
echo "content" | mail "test <test@mydomain.de>" -s "subject"

I think nextcloud already contacted the mail server successfully, then the mail server couldn’t find the email you inputted at settings > personal info, hence returning code 554.

Maybe try reading the log in the mail server?